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Blastomyces Antibody, Immunodiffusion

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Test Description
  • Contact: Serology (319) 335-4291
  • Test Request Form: Serology/Immunology
  • SHL OpenELIS Code: 599
Detection of Blastomyces-specific antibodies to determine recent/active infection
Panel Components
Reflex Test(s)
Samples with positive identification for Blastomyces specific antibodies will be forwarded to the Centers for Disease Control and Prevention (CDC) for complement fixation testing. The test will be listed as "Miscellaneous, Miscellaneous".
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
5 - 7 business days Immunodiffusion
Fee CPT Code(s)
$25.74 86612
Specimen Requirements
Specimen Type: Minimum 1 mL Serum
Patient Preparation:
Collection Instructions: Allow blood samples to clot at room temperature prior to centrifugation. Aseptically transfer serum to a tightly closing sterile container for storage.

Red Top Tube: centrifuge and transfer serum to a secondary tube
    Serum Separator Tube: collect 4 - 6 mL and centrifuge
      Shipping:
      Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
      Temperature and Stability: Refrigerate (2-8 degrees C); stable for up to 72 hours. Freeze (-20 degrees C); stable for more than 72 hours.
      Rejection Criteria: Specimens will be rejected if received under these conditions:

      Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected.
      Expected Results:
      Negative, A band
      Comments
      A positive result may suggest active or recent infection. The test is positive in about 80% of cases. Cross-reactions occur, especially with histoplasmosis. A negative test does not exclude blastomycosis. This is the screening assay. Positive results are confirmed by complement fixation.
      Alternate Names
      Fungal serology; Blastomyces dermatitidis Antibodies, Precipitin
      The University of Iowa

      State Hygienic Laboratory

      General inquires
      SHL Client Services
      319-335-4500 or 1-800-421-IOWA (4692)
      ask-shl@uiowa.edu

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