Test Detail

CDC Genotyping - Positive Cyclospora (May - September)

Performed	Method
Test Description		Contact: Diagnostic Microbiology (319) 335-4256 Test Request Form: Mycobacteriology/Mycology/Parasitology SHL OpenELIS Code:
Cyclospora Genotyping performed by the CDC for epidemiological purposes. No results report will be issued. Submission to the CDC with only known positive Cyclospora samples.
Coralville, 8 a.m. - 5 p.m., M-F	DNA Extraction, Real-Time Polymerase Chain Reaction (PCR), PCR Amplification, Illumina Sequencing
Fee
No charge.
Specimen Requirements
Specimen Type:	Stool (diarrheal) specimen. Minimum 1 mL volume. Stool preservative without formalin (i.e., Carey-Blair, Total Fix). Any fixative or transport media except for formalin. Specimens in no media/fixatives are also accepted.
Patient Preparation:	The patient should be instructed not to take any antacids, oily laxatives, or anti-diarrheal medications unless prescribed by the physician.
Collection Instructions:	Collect the specimen using a Stool Collection for Ova and Parasites Kit provided by SHL. The kit includes a specimen bag, an absorbent sheet, and one Total-Fix vial to preserve the stool specimen. Label vial with patient's name, unique identifier (e.g., date of birth), and date of collection. Pass the stool into a clean, dry plastic disposable container or place a large plastic bag or kitchen wrap over the toilet seat to catch the specimen. DO NOT MIX URINE OR WATER WITH THE STOOL SPECIMEN. Semi-formed stool: Using the spork built into the lid, add stool to the vial until the liquid reaches the red fill line. Any blood or mucous should be included. Liquid stool: Pour the stool into the vial up to the red fill line. Tighten the vial cap completely. A leaking specimen may be unsuitable for testing. Clean the outside of the vial with rubbing alcohol or soap and water if they are soiled. Check to make sure the patient name and date of collection are still readable. Wash hands thoroughly. Avoid contact with the Total-Fix solution inside the vial. If contact occurs, flush the affected area with water. Contact a physician immediately if irritation develops.
Shipping:
Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place in a biohazard bag. Seal the bag and place into the mailer along with a completed Test Request Form. Transport specimen at room temperature. Ship to the Coralville location. Preferably ship within 7 days of collection, but specimens within 6 months of collection (less than 6 months old) may also be sent.
Temperature and Stability:	Room Temperature (15-35 °C). If no media/fixative used, store specimens refrigerated (2-8 °C) prior to shipping.
Rejection Criteria:	Specimens will be rejected if received under these conditions: Formed stool, improperly collected specimens (e.g., rectal swabs), leaking specimen, multiple specimens within 24 hours, specimens containing barium, oil, water, or urine. Frozen specimens.
Expected Results:
Results will not be provided.
Comments
WARNING! DO NOT DRINK THE TOTAL-FIX SOLUTION IN THE COLLECTION KIT -- IT IS POISONOUS. If ingestion occurs, drink milk or water. Call the local Poison Control Center, your local physician, or the Iowa Poison Control Center day or night at 1-(800) 222-1222.