Test Detail
Diphtheriae Culture
Back to Test DirectoryTest Description |
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Bacterial culture and identification of Corynebacterium diphtheriae. PCR for diphtheria toxin gene. You MUST call the lab at 319/335-4335 before sending. | |||||
Performed | Avg. Turnaround Time | Method | |||
Centers for Disease Control and Prevention |
10 business days | Culture, Multi-target Polymerase Chain Reaction (PCR) | |||
Fee | CPT Code(s) | ||||
No charge | 87081 | ||||
Specimen Requirements | |||||
Specimen Type: | Throat, nasal, or wound swab, pseudomembrane or heart tissue, pure culture isolate. | ||||
Collection Instructions: |
Swab: Collect from patient and immediately place in Amies clear gel transport tube and store refrigerated (2 - 8 degrees C) until shipment. |
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Shipping: | |||||
Contact the SHL Reference Microbiology Lab at 319/335-4335 before sending a specimen. SHL will provide the CDC submission form and guidance for filling it out and shipping the sample directly to CDC. Samples must be received at CDC M - F. Throat, Nasal, or wound swab for culture and PCR: ship refrigerated overnight with refrigerated or frozen cold packs within 24-72 hours of collection. Specimens must arrive at 2-8 °C. Throat, nasal, or wound swab for PCR only (no culture): Ship frozen overnight with dry ice within 1 week of collection. Once frozen, do not allow swab to thaw. Pseudomembrane or heart tissue for culture only (no PCR): Ship refrigerated overnight with refrigerated or frozen cold packs within 24-48 hours of collection. Pure culture isolate for confirmation by culture and/or PCR: Ship refrigerated (2-8 °C) overnight with refrigerated or frozen cold packs OR ship ambient (15-25°C) overnight with room temperature cold packs. | |||||
Temperature and Stability: | Throat, nasal, or wound swab for PCR only (no culture): Freeze at -20°C or lower within 30 minutes of collection. Ship within 24-72 hours of collection. Throat, nasal, or wound swab for culture and PCR: Immediately place swab in an Amies clear gel transport tube and store within 30 minutes of collection refrigerated (2-8°C) until shipment. Ship within 24-72 hours of collection. Pseudomembrane or heart tissue for culture only (no PCR): Store within 30 minutes of collection refrigerated (2-8°C) until shipment. Ship within 24-48 hours of collection. Pure culture isolate for confirmation by culture and/or PCR: Maintain isolate on any agar or transport medium that supports the growth of Corynebacterium species. | ||||
Rejection Criteria: |
Specimens will be rejected if received under these conditions: Throat, nasal, or wound swab for culture and PCR, Pseudomembrane or heart tissue for culture only (no PCR): Specimens received outside of 2-8 °C, specimens received outside the specified transport time. Throat, nasal, or wound swab for PCR only (no culture): Samples received thawed or without dry ice. |
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Expected Results: | |||||
See attached CDC final report. | |||||
Comments | |||||
This testing is performed at the Centers for Disease Control and Prevention, Infectious Diseases Laboratories. This test and method will be named "Miscellaneous, Miscellaneous" on the SHL result report. | |||||
Alternate Names | |||||
C. diphtheria culture, Culture Corynebacterium diphtheriae |