| Test Description |
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Contact: Reference Microbiology
(319) 335-4335
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Test Request Form: CDC Submission Form. Call the lab at 319/335-4335 for form and instructions.
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SHL OpenELIS Code: 710
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| Bacterial culture and identification of Corynebacterium diphtheriae. PCR for diphtheria toxin gene. You MUST call the lab at 319/335-4335 before sending. |
| Performed |
Avg. Turnaround Time |
Method |
Centers for Disease Control and Prevention
|
10 business days |
Culture, Multi-target Polymerase Chain Reaction (PCR) |
| Fee |
CPT Code(s) |
| No charge |
87081 |
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| Throat, nasal, or wound swab, pseudomembrane or heart tissue, pure culture isolate. |
Swab: Collect from patient and immediately place in Amies clear gel transport tube and store refrigerated (2 - 8 degrees C) until shipment.
Pseudomembrane or Heart tissue: Collect from patient and place in physiological saline without formalin in a leak-proof, plastic container. Store refrigerated (2 - 8 degrees C) within 30 minutes of collection until shipment.
Isolates: Inoculate 5% sheep blood, chocolate, or trypticase soy agar (TSA) slant or plate and incubate aerobically at 35 - 37 degrees C until growth is observed. Store refrigerated (2 - 8 degrees C) until shipment.
Alternatively, collect colony growth from a 5% sheep blood, chocolate, or TSA plate within 24 hours of plate inoculation using a swab. Place the swab in a tube of Amies clear gel transport. Do not incubate the Amies clear gel transport. Store the inoculated transport tube refrigerated (2 - 8 degrees C) until shipment.
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| Store samples refrigerated (2 - 8 degrees C) within 30 minutes of collection. Ship swabs, pseudomembrane, and heart tissue within 24 - 72 hrs. of collection. Ship isolates within 24 - 48 hrs. of growth or transport inoculation. |
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| See attached CDC final report. |
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| Contact the SHL Micro Lab at 319/335-4335 before sending a specimen. SHL will provide the CDC submission form and guidance for filling it out and shipping the sample directly to CDC. Samples must be received at CDC M - F. Samples should be shipped overnight with frozen cold packs. |
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| This testing is performed at the Centers for Disease Control and Prevention, Infectious Diseases Laboratories. This test and method will be named "Miscellaneous, Miscellaneous" on the SHL result report. |
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| C. diphtheria culture, Culture Corynebacterium diphtheriae |
