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Hepatitis B Surface Antibody

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Test Description
  • Contact: Serology (319) 335-4291
  • Test Request Form: Serology/Immunology
  • SHL OpenELIS Code: 1061
Qualitative and quantitative determination of antibody to hepatitis B surface antigen.
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
5 - 7 business days Chemiluminescent Immunoassay (CLIA) - DiaSorin
Fee CPT Code(s)
$21.42 86706
Specimen Requirements
Specimen Type: Minimum 1 mL Serum
Collection Instructions: Blood should be collected aseptically by venipuncture, allowed to clot, and the serum separated from the clot as soon as possible.

Red Top Tube: centrifuge and transfer serum to a secondary tube.
    Serum Separator Tube: collect 4 - 6 mL and centrifuge.
      Shipping:
      Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
      Temperature and Stability: Refrigerate (2-8 degrees C); stable for up to 7 days. Freeze (-20 degrees C); stable for more than 7 days.
      Rejection Criteria: Specimens will be rejected if received under these conditions:

      Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected.
      Expected Results:
      Negative or Positive and value in mIU/mL
      Comments
      For a positive result: CDC guidelines currently state that a value of >=10 mIU/mL indicates recovery from acute or chronic hepatitis B virus infection or acquired immunity from HBV vaccination. This assay does not differentiate between a vaccine-induced immune response and an immune response induced by infection with HBV. For a negative result: CDC guidelines currently state that a value of <10.00 mIU/mL indicates a lack of recovery from acute or chronic hepatitis B or inadequate immune response to HBV vaccination.
      Alternate Names
      HBV surface antibody, HBsAb, Hepatitis B Immunity
      The University of Iowa

      State Hygienic Laboratory

      General inquires
      SHL Client Services
      319-335-4500 or 1-800-421-IOWA (4692)
      ask-shl@uiowa.edu

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