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Hepatitis B Surface Antigen

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Test Description
  • Contact: Serology (319) 335-4291
  • Test Request Form: Serology/Immunology
  • SHL OpenELIS Code: 1063
Qualitative detection of hepatitis B surface antigen.
Reflex Test(s)
Hepatitis B Surface Antigen Confirmation by Chemiluminescent Immunoassay (CLIA) - DiaSorin
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
5 - 7 business days Chemiluminescent Immunoassay (CLIA) - DiaSorin
Fee CPT Code(s)
$19.30 87340
Specimen Requirements
Specimen Type: Minimum 1 mL Serum
Collection Instructions: Blood should be collected aseptically by venipuncture, allowed to clot, and the serum separated from the clot as soon as possible.

Red Top Tube: centrifuge and transfer serum to a secondary tube.
    Serum Separator Tube: collect 4 - 6 mL and centrifuge.
      Shipping:
      Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
      Temperature and Stability: Refrigerate (2-8 degrees C); stable for up to 7 days. Freeze (-20 degrees C); stable for more than 7 days.
      Rejection Criteria: Specimens will be rejected if received under these conditions:

      Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected.
      Expected Results:
      Non Reactive, Reactive
      Comments
      A reactive test result indicates patient is presumed positive for HBsAg and a confirmatory test will follow (Hepatitis B Surface Antigen Confirmation by Chemiluminescent Immunoassay (CLIA) - DiaSorin). A non-reactive test result indicates patient is presumed negative for HBsAg. Tests of Public Health Significance (TOPHS): All tests submitted to SHL at no charge as Tests of Public Health Significance (TOPHS) must come from State of Iowa Department of Health and Human Services (HHS)-approved test sites, such as Community-Based Screening Services (CBSS) and Integrated Testing Services (ITS) sites. To qualify as TOPHS testing individuals must be unvaccinated for hepatitis B and meet one or more of the following criteria: 1. Men who have sex with men; 2. HIV-positive persons -- select "Previous related diagnosis/therapy" as an indicated risk; or 3. Persons who have ever injected drugs (PWID).
      Alternate Names
      HBsAg, Hep B surface antigen, HBV surface antigen, HBsAG Neutralization
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      State Hygienic Laboratory

      General inquires
      SHL Client Services
      319-335-4500 or 1-800-421-IOWA (4692)
      ask-shl@uiowa.edu

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