Test Detail
HIV Ag/Ab and Treponema pallidum Ab (Syphilis w/ VDRL) Panel
Back to Test Directory| Test Description |
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| HIV Ag/Ab: Screening test for the qualitative detection of HIV p24 antigen and antibodies to HIV-1 (Groups M and O) and HIV-2. Positive test results will be confirmed with a supplemental differentiation test. Treponema pallidum Antibody (Syphilis, with VDRL reflex): Treponemal assay for the qualitative determination of total antibodies to Treponema pallidum. In conjuction with non-treponemal laboratory tests and clinical findings, it may aid in the diagnosis of syphilis infection. | |||||
| Panel Components | |||||
| HIV Ag/Ab, Treponema pallidum Antibody (Syphilis, with VDRL reflex) | |||||
| Reflex Test(s) | |||||
| HIV-1/2 Antibody Differentiation Supplemental by Qualitative Enzyme Immunoassay (EIA). If the Treponema pallidum Antibody (Syphilis, with VDRL reflex) test is Positive or Equivocal, SHL will automatically reflex to the confirmatory nontreponemal test, Syphilis VDRL (Serum) by Flocculation with an end-point titer. Discordant results will be followed with a second treponemal test, Syphilis TPPA by Particle Agglutination. | |||||
| Performed | Avg. Turnaround Time | Method | |||
| Coralville, 8 a.m. - 5 p.m., M-F |
2 - 3 business days | Chemiluminescent Immunoassay (CLIA) - DiaSorin | |||
| Fee | CPT Code(s) | ||||
| $55.98. All tests submitted to SHL at no charge as Tests of Public Health Significance must come from State of Iowa Department of Health and Human Services-approved test sites such as Community-Based Screening Services and Integrated Testing Services sites. | 87389, 86780 | ||||
| Specimen Requirements | |||||
| Specimen Type: | Minimum 2 mL Serum | ||||
| Collection Instructions: |
Blood should be collected aseptically by venipuncture, allowed to clot, and the serum separated from the clot as soon as possible.
Red Top Tube: centrifuge and transfer serum to a secondary tube |
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| Temperature and Stability: | Refrigerate (2-8 degrees C); stable for less than 5 days. Freeze (-20 degrees C); stable for more than 5 days. | ||||
| Unacceptable Conditions: | Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. | ||||
| Expected Results: | |||||
| HIV Ag/Ab: Non Reactive, Reactive. Treponema pallidum Antibody: Negative, Equivocal, Positive. | |||||
| Shipping: | |||||
| Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location. | |||||
| Comments | |||||
| HIV Ag/Ab: A Reactive result indicates presence of HIV p24 antigen and/or HIV specific antibodies and a supplemental test will follow (HIV-1/2 Antibody Differentiation Supplemental by Qualitative Enzyme Immunoassay (EIA)). A Nonreactive result indicates no HIV p24 antigen or HIV specific antibodies present. Tests of Public Health Significance (TOPHS): All tests submitted to SHL at no charge as Tests of Public Health Significance (TOPHS) must come from State of Iowa Department of Health and Human Services (HHS)-approved test sites, such as Community-Based Screening Services (CBSS) and Integrated Testing Services (ITS) sites. To qualify as TOPHS testing individuals must must meet one or more of the following criteria: 1. Men who have sex with men; 2. Disproportionately impacted populations (as determined by Iowa HHS based on epidemiological data) -- Black/African American, Hispanic/Latino; 3. Persons who have ever injected drugs (PWID); 4. Persons who exchange sex for drugs or money; 5. Persons with an STI diagnosis within the last 12 months or someone who has a partner in another risk group (PWID, MSM, recent STI, exchange sex for drugs or money); or 6. Persons in need of confirmatory testing who have had reactive rapid HIV test results at an Iowa HHS-approved site -- select "Previous related diagnosis/therapy" as an indicated risk. Treponema pallidum Antibody (Syphilis, with VDRL reflex): Acceptable initial screening test for syphilis, as well as for monitoring treatment response in established syphilis. SHL follows the reverse algorithm for syphilis testing, meaning that we begin with a treponemal test, Treponema pallidum Antibody (Syphilis)(Serum). If this test is Positive or Equivocal, SHL will automatically reflex to the confirmatory nontreponemal test, Syphilis VDRL (Serum) with an end-point titer. Discordant results will be followed with a second treponemal test, Syphilis TPPA. Tests of Public Health Significance (TOPHS): All tests submitted to SHL at no charge as Tests of Public Health Significance (TOPHS) must come from Iowa Department of Public Health (IDPH)-approved test sites, such as Community-Based Screening Services (CBSS) and Integrated Testing Services (ITS) sites. To qualify as TOPHS testing individuals must must meet one or more of the following criteria: 1. Persons who have had signs or symptoms consistent with primary or secondary syphilis within the last 12 months; 2. Men who have sex with men; 3. Persons diagnosed with other STIs within the last 12 months; 4. Persons who exchange sex for drugs or money; 5. Persons with a sex partner in one of the above risk groups (e.g., signs/symptoms of primary or secondary syphilis, MSM); 6. Persons who have recently been treated for syphilis to monitor serologic response (titers) at intervals recommended by the CDC -- select "Previous related diagnosis/therapy" as an indicated risk; 7. Persons in need of follow up testing who have had questionable or incomplete syphilis test results (e.g., preliminary reactive syphilis test result from a blood or plasma center) -- select "Previous related diagnosis/therapy" as an indicated risk; or 8. Pregnant women -- select "Previous related diagnosis/therapy" as an indicated risk. | |||||
| Alternate Names | |||||
| HIV-1/HIV-2 Discriminatory Assay, HIV-1/2 Ag/Ab Combo, Multispot HIV-1/HIV-2 Rapid Test, Treponemal assay | |||||
