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HIV 1/2 Antibody Differentiation Supplemental

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Test Description
  • Contact: Serology (319) 335-4291
  • Test Request Form: Serology/Immunology
  • SHL OpenELIS Code: 834
Confirmatory and differential test for the detection of HIV antibodies in patient serum
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
2 - 3 business days Qualitative Enzyme Immunoassay (EIA)
Fee CPT Code(s)
$35.77 86701, 86702
Specimen Requirements
Specimen Type: Minimum 1 mL Serum
Collection Instructions: Allow blood samples to clot at room temperature prior to centrifugation. Aseptically transfer serum to a tightly closing sterile container for storage.

Red Top Tube: centrifuge and transfer serum to a secondary tube
    Serum Separator Tube: collect 4 - 6 mL and centrifuge
      Shipping:
      Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
      Temperature and Stability: Refrigerate (2-8 degrees C); stable for up to 7 days. Freeze (-20 degrees C); stable for more than 7 days.
      Rejection Criteria: Specimens will be rejected if received under these conditions:

      Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected.
      Expected Results:
      HIV-1 Antibody: Positive, Indeterminate, Negative, Invalid. HIV-2 Antibody: Positive, Indeterminate, Negative, Invalid. HIV Interpretation: HIV Negative, HIV Indeterminate, HIV-1 Indeteminate, HIV-2 Indeterminate, HIV-1 Positive, HIV-2 Positive, HIV-2 Positive with HIV-1 cross-reactivity, HIV Positive Untypable (undifferentiated), Invalid.
      Comments
      A nonreactive result indicates no antibodies present for HIV-1 or HIV-2. An HIV-1 Reactive result indicates the presence of HIV-1 antibodies only. An HIV-2 Reactive result indicates the presence of HIV-2 antibodies only. A reactive (undifferentiated) result indicates the presence of antibodies to HIV, but cannot be differentiated. An HIV-1 Indeterminate result indicates the patient is indeterminate for HIV-1 antibodies and supplemental testing for HIV nucleic acid is recommended.
      Alternate Names
      Geenius
      The University of Iowa

      State Hygienic Laboratory

      General inquires
      SHL Client Services
      319-335-4500 or 1-800-421-IOWA (4692)
      ask-shl@uiowa.edu

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