Test Detail
HIV Ag/Ab
Back to Test Directory| Test Description |
|
||||
|---|---|---|---|---|---|
| Screening test for the qualitative detection of HIV p24 antigen and antibodies to HIV-1 (Groups M and O) and HIV-2. Positive test results will be confirmed with a supplemental differentiation test. | |||||
| Reflex Test(s) | |||||
| HIV-1/2 Antibody Differentiation Supplemental by Qualitative Enzyme Immunoassay (EIA) | |||||
| Performed | Avg. Turnaround Time | Method | |||
| Coralville, 8 a.m. - 5 p.m., M-F |
2 - 3 business days | Chemiluminescent Immunoassay (CLIA) - DiaSorin | |||
| Fee | CPT Code(s) | ||||
| $33.14 | 87389 | ||||
| Specimen Requirements | |||||
| Specimen Type: | Minimum 1 mL Serum | ||||
| Collection Instructions: |
Blood should be collected aseptically by venipuncture, allowed to clot, and the serum separated from the clot as soon as possible.
Red Top Tube:centrifuge and transfer serum to a secondary tube |
||||
| Shipping: | |||||
| Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location. | |||||
| Temperature and Stability: | Refrigerate (2-8 degrees C); stable for up to 7 days. Freeze (-20 degrees C); stable for more than 7 days. | ||||
| Rejection Criteria: |
Specimens will be rejected if received under these conditions: Plasma, CSF. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected. |
||||
| Expected Results: | |||||
| Non Reactive, Reactive | |||||
| Comments | |||||
| A Reactive result indicates presence of HIV p24 antigen and/or HIV specific antibodies and a supplemental test will follow (HIV-1/2 Antibody Differentiation Supplemental by Qualitative Enzyme Immunoassay (EIA)). A Nonreactive result indicates no HIV p24 antigen or HIV specific antibodies present. Tests of Public Health Significance (TOPHS): All tests submitted to SHL at no charge as Tests of Public Health Significance (TOPHS) must come from State of Iowa Department of Health and Human Services (HHS)-approved test sites, such as Community-Based Screening Services (CBSS) and Integrated Testing Services (ITS) sites. To qualify as TOPHS testing individuals must must meet one or more of the following criteria: 1. Men who have sex with men; 2. Disproportionately impacted populations (as determined by Iowa HHS based on epidemiological data) -- Black/African American, Hispanic/Latino; 3. Persons who have ever injected drugs (PWID); 4. Persons who exchange sex for drugs or money; 5. Persons with an STI diagnosis within the last 12 months or someone who has a partner in another risk group (PWID, MSM, recent STI, exchange sex for drugs or money); or 6. Persons in need of confirmatory testing who have had reactive rapid HIV test results at an Iowa HHS-approved site -- select "Previous related diagnosis/therapy" as an indicated risk. | |||||
| Alternate Names | |||||
| HIV-1/HIV-2 Discriminatory Assay, HIV-1/2 Ag/Ab Combo, Multispot HIV-1/HIV-2 Rapid Test | |||||
