Test Detail
Legionella Multiplex
Back to Test Directory| Test Description |
|
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| Nucleic acid amplification by PCR to detect Legionella DNA. | |||||
| Performed | Avg. Turnaround Time | Method | |||
| Coralville, 8 a.m. - 5 p.m., M-F |
1 - 3 business days | Nucleic Acid Amplification by Polymerase Chain Reaction (PCR) | |||
| Fee | CPT Code(s) | ||||
| $69.97 | 87541 | ||||
| Specimen Requirements | |||||
| Specimen Type: | Bacterial isolate, Bronchial brushings, Bronchoalveolar Lavage (BAL), Sputum, Tracheal aspirate, Tissue specimen. PCR is a very sensitive test, and precautions should be taken to not cross-contaminate specimens (i.e., wear gloves and change before and after collecting specimen. Avoid contact with environmental surfaces). DO NOT FREEZE SPECIMEN. | ||||
| Collection Instructions: | Collect specimen using a sterile container. Sputum: If possible, have the patient rinse mouth and gargle with water prior to sputum collection. An early morning specimen is best. Instruct the patient not to expectorate saliva or postnasal discharge into container. Collect specimen resulting from deep cough in sterile screw-cap container. Satisfactory quality implies the presence of mucoid or mucopurulent material and is of greater significance than volume. Ideally, a sputum specimen should have a volume of 3-5 mL, although smaller quantities are acceptable if the quality is satisfactory. |
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| Temperature and Stability: | Refrigerate (2-8°C); DO NOT FREEZE; Overnight | ||||
| Unacceptable Conditions: | Frozen samples | ||||
| Expected Results: | |||||
| Legionella species detected, Legionella pneumophila (not serogroup 1) detected, Legionella pneumophila serogroup 1 detected, None Detected, Indeterminate | |||||
| Shipping: | |||||
| Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location. | |||||
| Comments | |||||
| Presence of Legionella DNA indicates likely infection. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or test-specific nucleic acid in concentrations below the level of detection by the test. | |||||
| Alternate Names | |||||
| Legionella pneumophila, Legionella species by PCR | |||||
