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Mumps Virus PCR

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Test Description
  • Contact: Molecular Biology and Virology (319) 335-4376
  • Test Request Form: Viral and Bacterial PCR
  • SHL OpenELIS Code: 576
Nucleic acid amplification by PCR to detect Mumps virus RNA
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
1 - 3 business days Nucleic Acid Amplification by Polymerase Chain Reaction (PCR)
Fee CPT Code(s)
$69.97 87798
Specimen Requirements
Specimen Type: Buccal/Oral swab. PCR is a very sensitive test, and precautions should be taken to not cross-contaminate specimens (i.e., wear gloves and change before and after collecting specimen. Avoid contact with environmental surfaces).
Collection Instructions: Collect specimen using a SHL Virus Isolation and Detection Kit (contains M4-RT viral transport medium and swabs) or equivalent viral transport medium.

Buccal (Oral) Swab: The buccal cavity is the space between the cheek and teeth. The parotid duct drains in the space near the upper molars. Massage the parotid gland area just in front of the ear and near the angle of the jaw for 30 seconds prior to collecting secretions on the swab. Swab the buccal cavity by sweeping the swab near the upper molar to the lower molar. Place swab in M4-RT viral transport medium and do not remove swab.
    Shipping:
    Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
    Temperature and Stability: Refrigerated (2-8°C); Overnight.
    Rejection Criteria: Specimens will be rejected if received under these conditions:

    Sample obtained on cotton-tipped, wooden-shafted or calcium alginate swabs. Samples not received in the correct temperature range of 2-8°C will be rejected.
    Expected Results:
    Not Detected, Detected, Indeterminate, Equivocal
    Comments
    Presence of Mumps virus RNA is indicative of mumps infection.
    Alternate Names
    Mumps PCR
    The University of Iowa

    State Hygienic Laboratory

    General inquires
    SHL Client Services
    319-335-4500 or 1-800-421-IOWA (4692)
    ask-shl@uiowa.edu

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