Test Detail
Orthopoxvirus (Monkeypox)
Back to Test Directory| Test Description |
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| The Orthopoxvirus, Conclusion, Qualitative Real-Time PCR test is intended for the qualitative detection of non-variola Orthopoxviruses and Monkeypox virus DNA using swabs from human pustular or vesicular rash specimens. | |||||
| Reporting Requirement | |||||
| Clinicians must report suspected monkeypox cases to the IDPH Center for Acute Disease Epidemiology (CADE), prior to collecting specimens. Tests ordered by a healthcare provider or clinical laboratory are posted to the SHL Web Portal upon completion, generally less than 12 hours from the time the specimen arrives at the lab. Healthcare providers will report results to the patient. The State Hygienic Laboratory will report results to IDPH. Positive specimens from SHL will be sent to the Centers for Disease Control and Prevention (CDC) for further studies. | |||||
| Performed | Avg. Turnaround Time | Method | |||
| Coralville, 8 a.m. - 5 p.m., M-F |
2 - 3 business days | Conclusion (Real-Time Polymerase Chain Reaction) | |||
| Fee | CPT Code(s) | ||||
| No charge (covered as a test of public health significance by IDPH). | 87593 | ||||
| Specimen Requirements | |||||
| Specimen Type: | Lesion Swab (two lesions, two swabs per lesion) | ||||
| Collection Kits: | Collection kits
are available for order on the SHL website: http://www.shl.uiowa.edu/kitsquotesforms/clinicalkit.xml. Select either the Monkeypox VTM kit or the Monkeypox Dry Swab Kit. NOTE: Keep VTM kits refrigerated. Healthcare facilities may use their own collection supplies as long as they meet the requirements (see unacceptable conditions below). Do not repurpose COVID collection kits for monkeypox testing. Specimens using COVID kits will be rejected. Testing materials and instrumentation for Monkeypox are not the same as those used for COVID testing. The contents of the specimen collection kits may be found here. |
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| Collection Instructions: | Select two lesions to swab and collect two swab specimens per lesion (total of four swabs). Swab each lesion vigorously. Place each swab into a separate viral transport media (VTM) or dry swab tube (do not put two swabs into a single tube). |
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| Packaging Instructions | The two collection tubes from the same lesion should be placed into a single biohazard bag with a single Test Request Form specific to that lesion (there will be one TRF per lesion for a total of two TRFs per patient). Keep specimens refrigerated until transported to the lab. |
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| Shipping: | |||||
| Ship refrigerated (on ice packs) via CDS courier to SHL (Category B). Contact CDS courier to schedule routine pick-up of specimens at http://cdsofiowa.com or by calling (515) 289-9990. | |||||
| Temperature and Stability: | Refrigerated (4 +/- 1 °C). Frozen: 30 days. | ||||
| Rejection Criteria: |
Specimens will be rejected if received under these conditions: Specimens meeting any of the following criteria will be rejected and not tested: |
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| Expected Results: | |||||
| Negative, Viral testing inconclusive, Test not done, Unable to rule out Variola virus, Presumptive positive identification of Orthopoxvirus, Presumptive positive identification of Non-variola Orthopox virus, Presumptive positive identification of variola virus, Presumptive positive identification of Varicella Zoster virus | |||||
| Comments | |||||
| Monkeypox is a rare disease caused by infection with the monkeypox virus. Monkeypox virus belongs to the Orthopoxvirus genus in the family Poxviridae. The Orthopoxvirus genus also includes variola virus (which causes smallpox), vaccinia virus (used in the smallpox vaccine), and cowpox virus. Monkeypox is unrelated to the chickenpox virus of the Herpesviridae family. Scientists at the Centers for Disease Control and Prevention (CDC) are tracking multiple cases of monkeypox that have been reported in several countries that have not reported monkeypox in the past, including the United States. The CDC is making the Jynneos vaccine available to clinical laboratory personnel who have a risk of exposure. Vaccination is voluntary. https://www.cdc.gov/poxvirus/monkeypox/clinicians/smallpox-vaccine.html According to the CDC, Monkeypox is rare and does not spread easily between people without close contact. The threat of monkeypox to the general U.S. population remains LOW as of 8/2/22. | |||||
| Alternate Names | |||||
| Monkeypox, Monkeypox Virus, Orthopoxvirus | |||||
