Test Detail
Shiga toxin-producing E. coli (STEC) (O157 and non-O157) Isolate Identification/Serotyping
Back to Test Directory| Test Description |
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| Determining the serogroup of Shiga toxin-producing Escherichia coli (STEC) (O157 and non-O157) isolates to assist epidemiological investigations. | |||||
| Panel Components | |||||
| Enteric Pathogen Identification/Serotyping | |||||
| Reflex Test(s) | |||||
| Enteric Pathogen Identification/Serotyping by Next Generation Sequencing, Shiga toxin - Producing E. coli (STEC) stx DNA Screen by Polymerase Chain Reaction (PCR), (Organism Extraction by Next Generation Sequencing: results not reported to facility) | |||||
| Performed | Avg. Turnaround Time | Method | |||
| Coralville, 8 a.m. - 5 p.m., M-F |
2 - 14 business days | Agglutination | |||
| Fee | CPT Code(s) | ||||
| No charge | 87147 | ||||
| Specimen Requirements | |||||
| Specimen Type: | A pure culture of Escherichia coli O157:H7 or other Shiga toxin-producing Escherichia coli (STEC). | ||||
| Collection Instructions: | Actively growing culture on suitable medium. | ||||
| Shipping: | |||||
| Ship to the Coralville location. Any CULTURE of Shiga toxin-producing E. coli (STEC) is considered a category A infectious substance under DOT and IATA regulations. Cultures of these organisms require specific packaging, labeling, and documentation before they can be transported to the laboratory for testing. Guidance for the proper packaging and shipping of category A infectious substances can be found at Hazardous Materials Regulations (HMR) 49 CFR 173.196. Specimens for shipment should be packaged following the basic triple packaging system consisting of (1) a primary container (a sealable specimen container) wrapped with absorbent material, (2) a secondary container (watertight, leak-proof), and (3) a rigid outer shipping package. Category A specimens must be packaged according to the Division 6.2 regulations with appropriate documentation. | |||||
| Temperature and Stability: | Refrigerated (2-8°C) or Room Temperature; stable for 3 days. | ||||
| Rejection Criteria: |
Specimens will be rejected if received under these conditions: Frozen specimens, mixed cultures, or non-viable organisms. |
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| Expected Results: | |||||
| Identification of organism and determination of its serotype | |||||
| Comments | |||||
| Required submission per Iowa Administrative Code {641} Chapter 1 | |||||
| Alternate Names | |||||
| O157 Rule Out, Sorbitol Negative E. coli serotyping, STEC serotyping, O157:H7 serotyping | |||||
