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West Nile Virus IgM Antibody

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Test Description
  • Contact: Serology (319) 335-4291
  • Test Request Form: Serology/Immunology
  • SHL OpenELIS Code: 680
Detection of IgM antibodies against West Nile Virus (active infection)
Performed Avg. Turnaround Time Method
Coralville, 8 a.m. - 5 p.m., M-F
5 - 7 business days IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA)
Fee CPT Code(s)
$33.59 86788
Specimen Requirements
Specimen Type: Minimum 1 mL Serum or 0.75 mL Cerebrospinal Fluid (CSF)
Collection Instructions: Allow blood samples to clot at room temperature prior to centrifugation. Aseptically transfer serum to a tightly closing sterile container for storage.

Red Top Tube: centrifuge and transfer serum to a secondary tube.
    Serum Separator Tube: collect 4 - 6 mL and centrifuge.
      CSF: Cerebrospinal Fluid (CSF) may be submitted for this test. Aseptically collect CSF from patient into a sterile, clear plastic tube with screw-top lid. Centrifuge if visible cellular debris is present.
        Shipping:
        Specimens should be stored and shipped under the same conditions. Wrap specimen container in absorbent material and place inside a biohazard bag. Transport specimen with cold pack. Ship to the Coralville location.
        Temperature and Stability: Refrigerate (2-8 degrees C); stable for up to 120 days. Freeze (-20 degrees C); stable for up to 1 year.
        Rejection Criteria: Specimens will be rejected if received under these conditions:

        Plasma. Grossly hemolyzed or lipemic samples as well as samples containing particulate matter or exhibiting obvious microbial contamination should not be tested. Specimens not received in the correct temperature ranges stated under "Temperature and Stability" will be rejected.
        Expected Results:
        Negative, Equivocal, Positive, Inconclusive
        Comments
        This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgM in serum specimens in which there is a clinical suspicion of West Nile virus infection. This test should not be used solely for quantitative purposes, nor should the results be used without correlation to clinical history or other data. Because other members of the Flaviviridae family, such as St. Louis encephalitis virus, show extensive cross-reactivity with West Nile virus, serologic testing specific for these species should be considered.
        Alternate Names
        West Nile, WNV
        The University of Iowa

        State Hygienic Laboratory

        General inquires
        SHL Client Services
        319-335-4500 or 1-800-421-IOWA (4692)
        ask-shl@uiowa.edu

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